[Nucleic Acids]

Transcription

Chapter 15

 


RNA types

1. Ribosomal RNA (rRNA): make up ribosomes & involved in translation. Produced in nucleolus.

2. Transfer RNA (tRNA): transport amino acids to ribosomes.

3. Messenger RNA (mRNA): copied from DNA, conveys information from chromosomes to ribosomes


Transcription: Process that converts DNA code of a gene into mRNA code.

Gene: Section of DNA within a chromosome that codes for the synthesis of one protein. There are roughly 20-30,000 genes on the 23 pairs of human chromosomes.

Only 1 side of DNA in a gene gets transcribed:

A. Sense Strand: Strand of DNA in a gene which has the same nucleotide sequence as the m-RNA (except with T instead of U).

B. Antisense Strand: Strand of DNA that is transcribed to create m-RNA. It is the template used for making m-RNA.

RNA polymerase: enzyme that initiates transcription by binding to promoter (TAC code on DNA). RNA polymerase reads the Antisense DNA Strand from 3'--->5'.

Promoter site: sequences in the DNA strand (TAC) which accept RNA polymerase and begin transcription

Elongation: mRNA bonds with DNA in small units, transcription bubble, adding bases Adenine to uracil & Guanine to cytosine, etc.. in the 5' to 3' direction (on the new m-RNA).

Termination: terminator or stop signal disengages RNA polymerase to end transcription.

[Transcription Diagram #1]

[Transcription Diagram #2]

Codon: sequence of 3 nucleotides on m-RNA that codes for one amino acid. Each amino acid has one to several different codons.

A Site with a good beginning Animation of Transcription (Needs Shockwave)


Much of DNA is non-coding nucleotide base sequences, not genes

Intron: part of gene (DNA or mRNA) that does not code for proteins. It must be removed before mRNA is exported to the ribosome.

Exon: coding part of DNA (or mRNA). The exon is what is translated at the ribosome during protein synthesis.

[How Introns are removed from m-RNA]

Slichter